Megazyme Fructanase Mixture (Ultrapure, recombinant, liquid)

SKU: E-FRLQPU

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Componentsexo-Inulinase (2,000 U/mL on kestose, at 40oC) and endo-Inulinase (100 U/mL on inulin at 40oC).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Content: 10 mL / 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: In 50% (v/v) glycerol plus 0.02% sodium azide
Physical Form: Solution
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity

Fructanase

EC Number

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

CAZy Family

GH32

CAS Number

9001-57-4,
9025-67-6,
37288-56-5

Synonyms

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

Source

Aspergillus niger

Concentration

endo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 10,000 U

Expression

Recombinant from Aspergillus niger

Specificity

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Unit Definition

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.

Temperature Optima

60oC

pH Optima

4.5

Application examples

Applications in carbohydrate research and in the food industry.

Method recognition

AOAC Method 997.08 and AOAC Method 2000.11

Megazyme Fructanase Mixture (Ultrapure, recombinant, liquid) Data Booklet

Download